The expression of APPL1 is altered by physiological or pathological factors such as obesity and hyperglycemia. APPL1 is widely expressed in adiponectin and insulin target tissues, such as skeletal muscle, liver endothelium, white adipose tissue, islet cells, ovary, etc. ShRNA set against Danio rerio APPL1 (NM_001128074)ĪPPL1 (Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1), formerly known as APPL or DIP13α, is an adaptor protein that binds directly to the adiponectin receptor. ShRNA set against Mouse APPL1 (NM_145221.2) The SureSilencing shRNA Plasmids are shipped on dry ice or cold packs. Each vector contains the shRNA under control of the U1 promoter and either the GFP gene, for the enrichment of transiently transfected cells, or the neomycin or puromycin resistance genes, for the selection of stably transfected cells. The SureSilencing trade shRNA Plasmids are designed to specifically knock down the expression of individual genes by RNA interference under either transient (with GFP) or stable transfection (for hygromycin, neomycin or puromycin-resistance) conditions after performance of the appropriate enrichment or selection procedures, respectively. ShRNA set against Human APPL1 (NM_012096.2) ShRNA set against Mouse Appl1(NM_145221.2) The sequences of 19~29mer shRNAi will be delivered with shRNA clones. The hairpin consists of a 7 base loop and 19~29 base stem optimized for the specific gene sequence. The hairpin consists of a 7 base loop and 19~29 base stem optimized for the specific gene sequence.Įach set contains 4 shRNA expression constructs. ShRNA set against Human APPL1(NM_012096.2)Įach set contains 4 shRNA expression constructs.
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